Dendrimer Signal Enhancement Independent of Label Type or Target Sequence

The following groups of images are photographs of x-ray films developed after probing for specific nucleic acid sequences with and without DNA dendrimers. The probing was done on dot blots that were prepared with identical amounts of target sequence where the plasmid mass of the sample is listed in the left column (plasmid mass) and the portion of the plasmid mass equal to the target mass is listed in the right column (target mass).

The following target sequences were used:

1) XRCC4- a human X-ray repair gene sequence

2) HIV-422 & HIV-520- specific for two positions in the HIV LTR

3) B2M-51 & B2M-151- human Beta-2-microglobin sequence (coding positions 51 & 151)

4)FSHr-51 & FSHr-251- human follicle stimulating hormone receptor (coding positions 51 & 251)

³²P indicates that the oligos used were end labeled with polynucleotide kinase and radioactive phosphorus

View ³²P Blots:
XRCC4 HIV-422 HIV-520 B2M-51 B2M-151 FSHr-51 FSHr-251

Digoxigenin indicates that the oligos were end labeled with terminal deoxynucleotidyl transferase using the Boehringer Mannheim Genius™ Kit.

View Dig Blots:
XRCC4 HIV-422 HIV-520 B2M-51 B2M-151 FSHr-51 FSHr-251

Biotin indicates that the oligos were labeled with psoralen-biotin using the Schleicher & Scheull Rad-Free Kit.

View Biotin Blots:
XRCC4 HIV-422 HIV-520 B2M-51 B2M-151 FSHr-51 FSHr-251

For Each Blot:

All 4 lanes were prepared with identical amounts of specific target

the 2 left lanes were probed using specific oligonucleotide alone

the right 2 lanes were probed using specific oligonucleotide with dendrimer

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Polyprobe ™ DNA Dendrimers and DNA Matrix Technology are covered under U.S. Patent No's 5,175,270 || 5,484,904, || 5,487,973 and patents pending.

Product Availability:
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